Journal: Frontiers in immunology

Article Title: Macrophage-Derived Protein S Facilitates Apoptotic Polymorphonuclear Cell Clearance by Resolution Phase Macrophages and Supports Their Reprogramming.

doi: 10.3389/fimmu.2018.00358

Figure Lengend Snippet: Figure 3 | Protein S (PROS1)-deficient macrophages display reduced reprogramming. Macrophages from Pros1fl/fl or LysMCre/+; Pros1fl/fl mice were isolated from peritoneal exudates 66 h after zymosan A injection and incubated for 24 h with vehicle or lipopolysaccharide (LPS, 1 µg/ml). Then, supernatants were collected and analyzed for their content of TNFα [(A), **P = 0.003], IL-6 (B), CCL3 [(C), *P = 0.02], and IL-10 [(D); *P ≤ 0.03] by standard ELISA. (E) IL-10 secretion by untreated or LPS-stimulated control and Pros1-cKO peritoneal macrophages, or by macrophages supplemented with either PROS1 (25 nM), apoptotic cells (AC) or both (AC + PROS1). Results represent the means ± SEM of at least three independent experiments. Two-way ANOVA, *P = 0.02, ***P ≤ 0.0001.

Article Snippet: reagents ELISA kits for mouse TNFα, IL-10, and IL-6 were obtained from Biolegend; a mouse CCL3 detection kit was obtained from R&D Systems.

Techniques: Isolation, Injection, Incubation, Enzyme-linked Immunosorbent Assay, Control

Journal: iScience

Article Title: Behenic acid alleviates inflammation and insulin resistance in gestational diabetes mellitus by regulating TLR4/NF-κB signaling pathway

doi: 10.1016/j.isci.2024.111019

Figure Lengend Snippet:

Article Snippet: Mouse MIP1α (Macrophage Inflammatory Protein 1 Alpha) ELISA Kit , ELK Biotechnology, China , Cat#ELK1116.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, RNA Extraction, SYBR Green Assay, CCK-8 Assay, Software